To identify and progress the most promising chemistry, and resolve the issues that inevitably arise, the right use of the right techniques at the right time is essential. This requires access to multiple techniques together with the expertise to run and interpret the results. This is a speciality at BioAscent and has developed by bringing together the use of biophysical, biochemical and cellular assays for over 150 molecular targets, providing corroborative and supportive data to create confidence in the results.
Our Biosciences team doesn’t just provide you with raw data. Our approach is to work with you to design and run the optimal assay cascade for your target, making a genuine scientific contribution to your project team. We have exceptional experience of triaging the output from hundreds of screening campaigns and are adept at identifying the most promising compounds to successfully kick-start medicinal chemistry programs. We can work with you to analyse screening data, identify false positives and PAINS (Pan-Assay Interference compounds), and generate orthogonal assay data to validate hits, elucidate compound mechanism of action, and drive rapid and cost-effective medicinal chemistry design, compound synthesis and testing cycles.
As part of our assay development/validation process we have developed a set of compounds known to cause interference in assays, so called PAINS activity, that can cause false positive results during screening (e.g. aggregators, redox cyclers, chelators etc.). We use these compounds during assay development to identify potential liabilities and can then either optimise the assay to minimise these ‘false positive’ effects, or in cases where these effects cannot be eliminated, we can put in place appropriate de-selection assays to quickly triage such compounds.
Key expertise includes:
Absorbance, luminescence, fluorescence, FP, HTRF, TR-FRET, Alphascreen
High-throughput cellular screening system
with acoustic dispensing capable of screening >150 plates per day
maintaining and drying plates in dry/low O2 atmosphere
software tools for secure Data Analysis in an industry standard, secure database
Posters & case studies